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KMID : 1142020180530040320
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Blood Research
2018 Volume.53 No. 4 p.320 ~ p.324
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Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings
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Ahmadi Abdolrahim
Kaviani Saeid
Yaghmaie Marjan
Pashaiefar Hossein
Ahmadvand Mohammad
Jalili Mahdi
Alimoghaddam Kamran
Eslamijouybari Mohammad
Ghavamzadeh Ardeshir
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Abstract
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Background: Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.
Methods: MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH).
Results: MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients.
Conclusion: Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.
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KEYWORD
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MALAT1, Chronic lymphocytic leukemia, qRT-PCR, FISH
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